by Staff Writers
Basel, Switzerland (SPX) Jun 10, 2015
Natural channel proteins are integrated into artificial membranes to facilitate the transport of ions and molecules. Researchers at the University of Basel have now been able to measure the movement of these channel proteins for the first time.
They move up to ten times slower than in their natural environment, namely the cell membrane. As reported in academic journal "Nano Letters", the results may prove useful to the ongoing development of new applications such as nanoreactors and artificial organelles.
The membranes of the cells in our bodies are only approximately 4 to 5 nanometers thick and consist of a complex mixture of lipids and specific membrane proteins, which also include channel proteins. This kind of cell membrane can be described as a fluid 2-D solution, in which the components are able to move laterally. These movements within the membrane are dependent on the flexibility and fluidity of the components and ultimately determine the functionality of the membrane.
Dynamic channel proteins
The researchers began by creating large membrane models with embedded and dyed channel proteins; they then put them on a glass surface and measured them using a single-molecule measuring method known as fluorescence correlation spectroscopy. All three channel proteins were able to move freely within the membranes of various thicknesses - this took up to ten times longer than in the lipid bilayers of their natural environment.
Flexibility is a necessity
This had already been described in theory. The researchers at the University of Basel have now been able to measure this in a practical experiment for the first time, demonstrating that the thicker the membrane, the slower the movement of the channel protein is in comparison to the movement of the actual polymers that form the membrane.
"This phenomenon is effectively a local decrease in fluidity caused by condensation of the polymers," explains lead author Fabian Itel.
In essence, however, the behavior of the channel proteins in the artificial membranes is comparable to that in their natural environment, the lipid bilayer, with the time scale of the movements being approximately ten times lower. The research project received funding from the Swiss National Science Foundation and the NCCR Molecular Systems Engineering.
University of Basel
Space Technology News - Applications and Research
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