Subscribe to our free daily newsletters
. 24/7 Space News .




Subscribe to our free daily newsletters



INTERNET SPACE
Clever use of mirrors boosts performance of light-sheet microscope
by Staff Writers
Woods Hole MD (SPX) Nov 29, 2017


Marine Biological Laboratory (MBL) Fellows Patrick La Riviere of University of Chicago, left, and Hari Shroff of the National Institute of Biomedical Imaging and Bioengineering with the diSPIM system (Dual-view Selective Plane Illumination Microscopy) co-developed by Shroff's laboratory and Applied Scientific Instrumentation. Note the two objectives mounted at right angles above the sample. A light sheet is created from one objective and imaged through the other objective. A stack of images is created by moving the light sheet through the sample. Especially useful for live-cell imaging and developmental biology, the system provides rapid 3-D imaging with low phototoxicity to samples.

Using a simple "mirror trick" and not-so-simple computational analysis, scientists affiliated with the Marine Biological Laboratory (MBL) have considerably improved the speed, efficiency, and resolution of a light-sheet microscope, with broad applications for enhanced imaging of live cells and embryos.

MBL Fellows Hari Shroff of the National Institute of Biomedical Imaging and Bioengineering (NIBIB) and Patrick La Riviere of the University of Chicago, with lead author Yicong Wu of NIBIB, report on their technique this week in Nature Communications.

"In one sense, it couldn't be easier," Shroff says of the team's technique. Instead of growing their biological samples on glass cover slips, they used mirrored cover slips, which are commercially available. They then mounted the samples on a microscope invented in Shroff's lab (the diSPIM), which has two objectives providing perpendicular views of the sample (see photo).

In the typical diSPIM set-up, one objective transmits a thin sheet of light to the sample while a camera behind the second objective collects the image. The objectives then switch roles: One illuminates a thin section of the sample in the perpendicular direction while the other objective images it. With mirrored cover slips, however, the transmitted light (as well as the fluorescence it produces in the sample) is reflected off the mirror, so the two objectives can simultaneously collect four complementary views of the sample.

This doubles the speed of the microscope and markedly improves its efficiency at collecting light, which is useful for imaging fast-moving biological processes and samples with low light.

But collecting more information faster is only half the battle: It then needs to be computationally resolved to produce a image. La Riviere led the team in developing an algorithm to fuse the views and optimize spatial resolution in all three dimensions (x, y, and z).

"The computation is really enabling for this technique," La Riviere says. "While the mirror multiplies the information captured by the cameras, it also introduces some contamination that would not normally be there. What we were able to show is by properly modeling the process - basically, by converting the microscope into mathematics - we could effectively remove that contamination and recover all the information (imaged by the cameras)."

The team demonstrated the technique's applicability by imaging a variety of live samples, including microtubule, mitochondrial, membrane, and Golgi dynamics in cells and calcium activity in nematode embryos.

"The ongoing collaboration between Hari Shroff and Patrick La Riviere to innovate at the cutting edge of light microscopy continues a long tradition at the MBL of developing new and exciting ways to examine the fundamental processes of cellular life," says David Mark Welch, MBL Director of Research.

Over the past year, scientists at the MBL have been using a diSPIM system generously on loan from Applied Scientific Instrumentation, Inc. As part of the MBL's strategic initiative to advance innovation in biological imaging and image analysis, the MBL will purchase a diSPIM system by 2018 that will be available for use by all resident and visiting scientists and students.

Research Report: Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy.

INTERNET SPACE
Photopolymerization-triggered molecular motion for flexible liquid crystal display
Tokyo, Japan (SPX) Nov 21, 2017
With current 2D techniques, one typically irradiates a liquid crystal film that contains added photoresponsive dye molecules, with uniform polarized light. This controls the net liquid crystal alignment via the interaction of the dye dipole and the polarization axis of light. The drawback with these systems is the need for adding strong dyes, which can discolor or degrade optical and stability p ... read more

Related Links
Marine Biological Laboratory
Satellite-based Internet technologies


Thanks for being here;
We need your help. The SpaceDaily news network continues to grow but revenues have never been harder to maintain.

With the rise of Ad Blockers, and Facebook - our traditional revenue sources via quality network advertising continues to decline. And unlike so many other news sites, we don't have a paywall - with those annoying usernames and passwords.

Our news coverage takes time and effort to publish 365 days a year.

If you find our news sites informative and useful then please consider becoming a regular supporter or for now make a one off contribution.

SpaceDaily Contributor
$5 Billed Once


credit card or paypal
SpaceDaily Monthly Supporter
$5 Billed Monthly


paypal only

Comment using your Disqus, Facebook, Google or Twitter login.

Share this article via these popular social media networks
del.icio.usdel.icio.us DiggDigg RedditReddit GoogleGoogle

INTERNET SPACE
Does the Outer Space Treaty at 50 need a rethink

NASA to send critical science, instruments to Space Station

Can a magnetic sail slow down an interstellar probe

SSL Selected to Conduct Power and Propulsion Study for NASA's Deep Space Gateway Concept

INTERNET SPACE
Flat-Earther's self-launch plan hits a snag

Mechanisms are critical to all space vehicles

SSTL ships CARBONITE-2 and Telesat's LEO-1 for PSLV launch

Russia loses contact with satellite after launch from new spaceport

INTERNET SPACE
Earthworms can reproduce in Mars-like soil

Gadgets for Mars

Ice shapes the landslide landscape on Mars

Winds Blow Dust off the Solar Panels Improving Energy Levels

INTERNET SPACE
Nation 'leads world' in remote sensing technology

China plans for nuclear-powered interplanetary capacity by 2040

China plans first sea based launch by 2018

China's reusable spacecraft to be launched in 2020

INTERNET SPACE
UK space launch program receives funding boost from Westminster

Need to double number of operational satellites: ISRO chief

Space Launch plans UK industry tour

Astronaut meets volcano

INTERNET SPACE
Borophene shines alone as 2-D plasmonic material

3rd SES bids farewell to ANGELS satellite

Booming life for 'PUBG' death-match computer game

New way to write magnetic info could pave the way for hardware neural networks

INTERNET SPACE
First known interstellar visitor is an 'oddball'

Lava or Not, Exoplanet 55 Cancri e Likely to have Atmosphere

Images of strange solar system visitor peel away some of the mystery

Familiar-Looking Messenger from Another Solar System

INTERNET SPACE
Pluto's hydrocarbon haze keeps dwarf planet colder than expected

Jupiter's Stunning Southern Hemisphere

Watching Jupiter's multiple pulsating X-ray Aurora

Help Nickname New Horizons' Next Flyby Target




Memory Foam Mattress Review
Newsletters :: SpaceDaily :: SpaceWar :: TerraDaily :: Energy Daily
XML Feeds :: Space News :: Earth News :: War News :: Solar Energy News






The content herein, unless otherwise known to be public domain, are Copyright 1995-2017 - Space Media Network. All websites are published in Australia and are solely subject to Australian law and governed by Fair Use principals for news reporting and research purposes. AFP, UPI and IANS news wire stories are copyright Agence France-Presse, United Press International and Indo-Asia News Service. ESA news reports are copyright European Space Agency. All NASA sourced material is public domain. Additional copyrights may apply in whole or part to other bona fide parties. All articles labeled "by Staff Writers" include reports supplied to Space Media Network by industry news wires, PR agencies, corporate press officers and the like. Such articles are individually curated and edited by Space Media Network staff on the basis of the report's information value to our industry and professional readership. Advertising does not imply endorsement, agreement or approval of any opinions, statements or information provided by Space Media Network on any Web page published or hosted by Space Media Network. Privacy Statement