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Scientists create successful mass production system for bioengineered livers by Staff Writers Cincinnati OH (SPX) Dec 18, 2017
Researchers report creating a biologically accurate mass-production platform that overcomes major barriers to bioengineering human liver tissues suitable for therapeutic transplant into people. A team led by the Cincinnati Children's Center for Stem Cell and Organoid Medicine (CuSTOM) and Yokohama City University in Japan report their findings Dec. 5 in Cell Reports. The study's lead investigator is Takanori Takebe, MD, a physician/investigator at both institutions. The current study overcomes what have been nagging challenges for researchers working to bioengineer human organs that are safe and effective for therapeutic use, according to Takebe. The new process allows researchers to bioengineer single batches of up to 20,000 genetically matched, three-dimensional and highly functional liver micro-buds. When combined, the batch has a sufficient quantity of liver cells and size feasibility for transplant into a person with liver failure, or for drug testing. The liver tissues were also generated entirely from human induced pluripotent stem cells (iPSCs), making the process free of animal feeder byproducts used to make cells for research purposes - a barrier to the cells being used therapeutically. "Because we can now overcome these obstacles to generate highly functional, three-dimensional liver buds, our production process comes very close to complying with clinical-grade standards," explains Takebe. "The ability to do this will eventually allow us to help many people with final-stage liver disease. We want to save the lives of children who need liver transplants by overcoming the shortage of donor livers available for this." The researchers stress continued research and refinement of their process is required before initial clinical trials could begin. Takebe estimates this might occur in the next two to five years.
Liver by Design To help overcome the biological challenge of animal-product feeder cells in the current study, Takebe and team used their fine-tuned formula of genetic and molecular components to generate the liver tissues in custom-designed, U-shaped bottom micro-well cell plates. The plates use a combination of chemistry techniques to form a finely structured film inside the micro-wells, designed to nurture the developing liver buds. But prior to this step, the researchers started mass production by initially using the donor-derived iPSCs to grow three critical types of liver progenitor cells needed to generate healthy livers. These include hepatic endoderm cells and both endothelial and septum mesenchyme cells. Study data show this generates robust and highly functional progenitor cells that are placed into the custom-designed, film-coated micro-wells. The progenitor cells then engage in high levels of molecular cross-communication to form into self-organizing, three-dimensional liver buds. The authors write that after they generated over 20,000 (liver) micro spots per well capable of reaching a scalable, therapeutically viable level, they tested the functionality of the three-dimensional tissues in genetically programmed mouse models of liver disease. The animals were rescued from liver disease by the highly functional organs.
New York NY (SPX) Dec 06, 2017 Through a few clever molecular hacks, researchers at Columbia University Medical Center have converted a natural bacterial immune system into a microscopic data recorder, laying the groundwork for a new class of technologies that use bacterial cells for everything from disease diagnosis to environmental monitoring. The researchers modified an ordinary laboratory strain of the ubiquitous hu ... read more Related Links Cincinnati Children's Hospital Medical Center Space Medicine Technology and Systems
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